LiTone XL Light-sheet Microscope
A versatile tool for imaging large cleared tissue with extraordinary resolution
The LiTone XL light-sheet microscope is designed to image large samples in 3D with resolution and speed. This powerful platform utilizes the Line Bessel Sheet (LBS) technology and features a unique 4-side illumination configuration providing the most uniform sample illumination in the market. The LiTone XL microscope is equipped with refractive index (RI) correction optics adjustable from 1.33 to 1.57 to ensure the best imaging quality in various immersion medium. The exchangeable sample chamber can host samples with size over 2cm.
Key Features
LBS light-sheets and RI correction optics
for the best performance. Resolution up to 500nm.
Unique four-side illumination
significantly increases the illumination depth and uniformity especially in large cleared samples.
Capable of long-term imaging for living embryos
LiTone XL is designed for cleared tissue imaging but is also a proven platform for imaging live Zebrafish or Drosophila embryos.
Smart and user-friendly LitScan software
with rapid data processing, built-in 3D rendering, multi-position acquisition auto-stitching and deconvolution.
Compact bench-top design
with built-in vibration isolation. No air-table is required.
The Line Bessel Sheet (LBS) technology
The sophisticated LBS design is crafted to be much thinner and longer than traditional Gaussian light sheets, making the LiTone LBS not only powerful in its low rate of photo toxicity and high imaging speed, but also providing superior 3D resolution and signal to noise ratio owing to its much-improved sectioning ability. The longer LBS also means data size is significantly reduced compared to methods that require a scanning light sheet.
Increased Depth of Penetration and Even Illumination Via 4-beam Scanning
LiTone XL generates 4 LBS light sheets with equal power that can selectively enter the sample from all four orthogonal sides to maximize FOV and reduce shadowing effects. Even hard to image samples like dense heart material (above) can be imaged with greater uniformity, improving accuracy of quantitative results. The system accepts a range of detection objectives including glycol/water immersion objectives or air objectives from 4x to 25x. The system features an adjustable optical system that corrects immersion RI from 1.33 to 1.57 when air objectives are used.
Increased Depth of Penetration via 4-side Illumination
LiTone XL generates 4 LBS light sheets with equal power that enter the sample from all four orthogonal sides to maximize FOV and reduce shadowing effect. The system accepts a range of detection objectives including glycol/water immersion objectives or air objectives from 4x to 25x. The system features an adjustable optical system that corrects immersion RI from 1.33 to 1.57 when air objectives are used.
Various selections of sample bath and sample holders
A range of reusable sample holders and immersion baths are available to provide optimal handling for a wide variety of samples, and refractive index media. Supports 4x, 10x and 25x objectives (others supported upon request).
Effortless Sample Loading
Unlike many traditional light sheet systems, the inverted configuration allows for easy loading of samples using the wide range of sample holders to securely mount samples for imaging.
On-the-fly data processing
Handling large imaging data is always dreadful, especially for large cleared samples where TBs of raw data need to be processed, transferred and stored.
Our latest LitScan control and analysis software offers built-in 5D data rendering package that allows on-the-fly 3D viewing and sophisticated movie rendering by a few clicks right after the data is taken into the memory.
LitScan data is compatible OMERO data standards for easy importation to your favorite 3rd party analysis packages
Smart tiling and multi-position acquisition
LiTone XL offers advanced tiling and multi-position functions to boost the efficiency in operation.
Smart tiling enables imaging of a large FOV without compromising the resolution. Users are guided through a simple and smart parameter setting process and the data is stitched and fused using our patented algorithm with efficiency and accuracy.
Multi-position functionality images multiple sample sites in one scan sequence thus significantly increasing the efficiency, particularly in organoid and embryo imaging experiments.
Data analysis support
LiT provides general and specialized data analysis services for delivering publication ready data, and offers comprehensive data analysis algorithms and methods for customized data analysis made to your specifications. Our rendering pipeline can be generalized for different data analysis demands and batch data processing.
Protein analysis
Vessel tracing
Brain region analysis
LiT supports data analysis including: