Whole brain macro- and micro-vasculature at sub-micron resolution
Vasculature
Detail, consistency and flexibility in one workflow
High-resolution volumetric imaging
Resolve fine structures across intact cleared tissues without physical sectioning.
Uniform imaging across dense samples
More consistent illumination with fewer dark regions and less striping.
Flexible sample handling
Adapt to different specimens, clearing methods, immersion media and imaging scales.
Integrated workflow
Move from acquisition and processing to 3D rendering and downstream analysis.
The optics behind the image
LiT was founded around patented Line Bessel Sheet technology. XL2 combines it with four-side illumination, refractive-index correction and a range of objectives.
Line Bessel Sheet
Long, thin light sheets for high-resolution imaging with reduced side-lobe background
Four-side illumination
More uniform illumination across dense cleared samples with less striping and shadowing
RI correction optics
Adjustable refractive-index correction from 1.33 to 1.58 for different clearing media
Multiple objectives
Flexible magnification from whole-sample overview to fine structural detail
Proven in practiceDemonstrated on intact tissue, organs and whole organisms
Cleared mouse forelimb imaged by the LiT XL2 light-sheet system
Cleared organs & tissues
Whole mouse kidney imaging with glomeruli quantification
Cleared organs & tissues
From acquisition to analysis-ready 3D data
Acquire
Control acquisition and imaging.
Process
Stitching, de-striping and background subtraction.
Render
Build and visualize 3D volumes.
Export
Prepare data for connected analysis tools.
Analyze
Render in 3D or export to Imaris or Amira
SpecificationsSystem specifications
Confirmed system parameters. Settings from individual published examples are not system specifications.
Objectives:4× / 5× / 10× / 16× / 25×
RI correction:Adjustable, 1.33–1.58
Illumination:Four-side Line Bessel Sheet illumination
Sample handling:Reusable, exchangeable sample bath;Holders for different sample sizes;Multiple mounting configurations
Sample size:2.5 cm × 2.5 cm × 2 cm, or 6.3 cm × 2.5 cm × 2 cmCustomization available for larger samples
Theoretical resolution:~310nm–887nm, wavelength dependent
System format:Compact benchtop design;No vibration table required under standard use;Vibration isolation may be useful at high magnification
How XL2 compares
Illumination approach
Dense-sample imaging
Refractive-index flexibility
Magnification range
Sample handling
System format
Processing workflow
XL2
Four-side Line Bessel Sheet illumination
Designed for more uniform illumination with reduced dark regions and striping
Adjustable RI correction from 1.33 to 1.58
Magnification range:4× / 5× / 10× / 16× / 25× objectives
Reusable sample baths and multiple holder configurations
Compact benchtop design
Acquisition, stitching, processing and 3D rendering supported through the LiT workflow
Typical alternatives
Commonly dual-side or multi-sheet illumination
Performance depends on illumination geometry and sample density
RI support varies by system
Objective options vary by platform
System-specific chambers and mounting workflows
Footprint and installation requirements vary
Often distributed across acquisition and downstream software tools
Support for complex imaging workflows
Setup & training
Installation, setup and system training.
Sample & imaging guidance
Clearing, mounting and acquisition support.
Data & analysis
Processing and analysis support.
We work alongside you at every stage, from setup to final results
Dr. Anna Keller
”
2023
If > 10
Featured Inhaled SARS-CoV-2 vaccine for single-dose dry powder aerosol immunization. Nature 624, 630–638
2023
If > 10
Transcriptional repression of beige fat innervation via a YAP/TAZ-S100B axis. Nat. Commun. 14, 7102
2023
If > 10
Lymphatic vessels invade bone in disease but are absent in health and regeneration." Cell, 189, 17, 5449-5465
